NovaSil® columns feature an advanced proprietary bonding technology that creates a unique, tree-like polymerized silica surface structure, providing superior chemical stability and an extended working pH range of 1–12. This innovative design enhances the durability of the silica surface and allows the column to be used with 100% aqueous mobile phases without degradation—ideal for both hydrophilic and hydrophobic analytes. The hydrophobicity of each NovaSil® phase depends on its bonding and endcapping chemistry, where multiple bonded phases result in higher carbon loads, ensuring stronger hydrophobic interactions and enhanced retention characteristics.Separation on NovaSil® columns occurs through a combination of penetration and adsorption mechanisms, providing excellent selectivity and resolution for a wide variety of compounds. Engineered for maximum batch-to-batch and column-to-column reproducibility, NovaSil® delivers consistent and reliable results across analytical applications, making it the preferred choice for laboratories that demand performance, precision, and long-term stability in their HPLC separations.