01) Paracetamol IP Assay
Column:
ChromX® C18 5µm, 250x4.6mm, P/N:CHI18502S045-0
Mobile Phase:
85%phosphate Buffer pH 4.0/ 7% ACN
Flow Rate:
1.0 ml/min
Column Oven:
40°C
UV:
215nm
Sample:
0.3mg/mL Paracetamol Standard
Chromatogram showing sharp peak at retention time ~5.1 minutes
Excellent peak shape and resolution
02) Favipiravir IP Assay
Column:
ChromX® C18 5µm, 250x4.6mm, P/N:CHI18502S045-0
Mobile Phase:
77%0.1% OPA/53% ACN
Flow Rate:
1.0 ml/min
Column Oven:
25°C
UV:
225nm
Sample:
5mg/100mL Favipiravir Standard
Chromatogram showing well-defined peak at retention time ~4.8 minutes
High sensitivity and selectivity
03) Cefixime Assay USP
Column:
ChromX® C18 (2) 4µm, 125x4.6mm, P/N: CHI18402S45-2
Mobile Phase:
25 volume of ACN and 75 volume of tetrabutylammonium
hydroxide solution prepared by diluting 25ml of 0.4M tetrabutylammoniumhydroxide
solution to 1000 ml with water and adjusted pH to 6.6 with OPA
Flow Rate:
1.4 ml/min
Column Oven:
40°C
UV:
254nm
Injection Volume:
10µL
Sample:
Cefixime Reference Solution
Chromatogram showing two distinct peaks (Imp-E and Cefixime)
Excellent separation and baseline resolution
Analytical Results Summary
| ID | Name | Ret. Time | Area | Height | Area% | Theoretical Plate# | Tailing Factor | Resolution | RRT |
|---|---|---|---|---|---|---|---|---|---|
| 1 | Imp-E | 9.100 | 2768481 | 165678 | 25.282 | 6708 | 1.27 | 0.00 | 1.0 |
| 2 | Cefixime | 10.172 | 8181977 | 385020 | 74.718 | 4330 | 1.73 | 2.02 | 1.0 |
| 10950405 | 630598 | ||||||||
04) Ranitidine hydrochloride RS IP
Column:
ChromX® C18-AQ 3.5µm, 100X4.6mm
Mobile Phase:
A- 2% ACN / 98% buffer (0.05M KH2PO4 pH 7.1)
B- 22% ACN / 78% buffer (0.05M KH2PO4 pH 7.1)
Flow Rate:
1.5 ml/min
Gradient:
| Time | %A | %B |
|---|---|---|
| 0 | 100 | 0 |
| 10 | 0 | 100 |
| 15 | 0 | 100 |
| 16 | 0 | 100 |
| 20 | 100 | 0 |
Column Temp:
Ambient
UV:
230nm
Injection Volume:
10µL
Sample:
Reference solution b
(Ranitidine HCl + impurity J)
Chromatogram showing two distinct peaks (Imp-E and Cefixime)
Excellent separation and baseline resolution
05) Analysis of Clrotimazol
Column:
ChromX® C8 5µm, 150X4.6mm, P/N:CH085015046-0
Mobile Phase:
A-1 gm K2HPO4 + 0.5g of tetrabutyammonium
hydrogen in 1000ml water, B- ACN
Flow Rate:
1.0 ml/min
Gradient:
| Time | %A | %B |
|---|---|---|
| 0 | 75 | 25 |
| 3 | 75 | 25 |
| 25 | 20 | 80 |
| 30 | 20 | 80 |
| 31 | 75 | 25 |
| 35 | 75 | 25 |
Column Oven
40°C
UV:
210nm
Sample:
Clrotimazol reference solution containing Imp-A,B &
Chromatogram showing two distinct peaks (Imp-E and Cefixime)
Excellent separation and baseline resolution